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Journal of Lipid Research, Vol. 1, 48-52, October 1959
Copyright © 1959 by Lipid Research, Inc.

Quantitative glass paper chromatography: phosphatidyl choline and sphingomyelin

James E. Muldrey , O. Neal Miller , and James G. Hamilton

Department of Biochemistry and the Nutrition and Metabolism Research Laboratory of the Department of Medicine, Tulane University School of Medicine, New Orleans 12, Louisiana

A rapid chromatographic procedure was developed for the separation of sphingomyelin, phosphatidyl choline, phosphatidyl ethanolamine, phosphatidyl serine, phosphatidyl inositol, and free fatty acids on glass paper coated with sodium silicate. In addition, phosphatidyl choline and sphingomyelin were determined quantitatively by densitometry of the charred chromatogram, which was obtained by spraying the developed chromatogram with sulfuric acid and heating in an oven. The separation of phosphatides on sodium silicate-treated glass paper is more rapid than on silicic acid-impregnated paper, and the former is simpler to prepare. Preliminary application of this quantitative technique to human serum indicates that it may have a wide adaptability for the determination of phospholipids in natural products.

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