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Journal of Lipid Research, Vol. 1, 60-65, October 1959
Copyright © 1959 by Lipid Research, Inc.

Positional distribution of saturated and unsaturated fatty acids on egg lecithin

N. H. Tattrie

Division of Applied Biology, National Research Council, Ottawa 2, Canada

A method is described to determine the position of fatty acids on lecithin. Purified egg lecithin is enzymatically hydrolyzed to the corresponding mixture of ,ßbeta;-diglycerides with lecithinase D. Myristic acid is incorporated into the ' position of the diglycerides and the resulting triglycerides are hydrolyzed with pancreatic lipase, which specifically cleaves fatty acids from the and ' positions. Palmitic, myristic, and stearic acids were freed by the pancreatic lipase, proving that the saturated acids are on the ' position, while the unsaturated fatty acids occupy the ßbeta; position of egg lecithin. Since snake venom lecithinase A removes the unsaturated acids from lecithin, the site of hydrolysis must be at the ßbeta; position of lecithin.

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