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Journal of Lipid Research, Vol. 1, 90-96, October 1959
Copyright © 1959 by Lipid Research, Inc.

Metabolism of adipose tissue: incorporation of isoleucine carbon into lipids by slices of adipose tissue

D. D. Feller and E. Feist

Radioisotope Service, Veterans Administration Hospital, and the Department of Medicine of the University of Washington School of Medicine, Seattle 8, Washington

Slices from adipose tissue and liver were incubated with uniformly labeled C¹⁴-isoleucine and the respiratory carbon dioxide, fatty acids, and nonsaponifiable lipids were isolated and analyzed for radioactivity. Organic acids were qualitatively identified by chromatography and autoradiography. Adipose tissue oxidized isoleucine to carbon dioxide at a rate greater than that of liver. Conversion to nonsaponifiable lipids was small for both tissues. Recovery of C¹⁴ from isoleucine C¹⁴ in fatty acids of adipose tissue was 50 to 100 times greater than recovery in liver slices. C¹⁴ was recovered in acetate, propionate, methylmalonate, and -methylacetoacetate in adipose tissue after incubation with isoleucine. The role of acetate, propionate, methylmalonate, -methylacetoacetate, and isoleucine in biosynthesis of fatty acids in adipose tissue is discussed.

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