J. Lipid Res. Neurobiology of Lipids (ISSN1683-5506)
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Journal of Lipid Research, Vol. 1, 199-202, April 1960
Copyright © 1960 by Lipid Research, Inc.

Titration of free fatty acids of plasma: a study of current methods and a new modification

David L. Trout , E. Harvey Estes Jr. , and Samuel J. Friedberg

Central Reference Laboratory, Veterans Administration Hospital, Durham, North Carolina, and Department of Medicine, Duke University Medical Center, Durham, North Carolina

Dole's titrimetric method for determination of the free fatty acids of plasma has been modified to improve its specificity. The fatty acid solution which is to be titrated is first washed with 0.05 per cent H2SO4 and thus freed of lactic acid and of an acetone-insoluble material which interferes. With this change, the Dole procedure yields results which agree well with those of Gordon's method.

Submitted on January 9, 1960


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