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Journal of Lipid Research, Vol. 1, 214-220, April 1960
Copyright © 1960 by Lipid Research, Inc.

Fat metabolism in higher plants: XIII. phosphatidic acid synthesis and diglyceride phosphokinase activity in mitochondria from peanut cotyledons

Clive Bradbeer and P. K. Stumpf

Department of Biochemistry, University of California, Davis, California

A phosphatidic acid is the major lipid to become labeled when Pi³² (inorganic orthophosphate labeled with P³²), under conditions of oxidative phosphorylation, or ATP³² is fed to mitocholine and phosphatidylethanolamine in the mitochondria obtained a much higher level of P³², the only cofactor required was Mg⁺⁺. The stimulation of phosphatidic acid synthesis by an ,ßbeta;-diglyceride provided support for the view that this synthesis is due to diglyceride phosphokinase activity in the mitochondria. Evidence is also presented that this enzyme preparation is capable of phosphorylating -monoglycerides with the formation of monoacyl phosphatidic acids. When Pi³² was added to slices of peanut cotyledons, the phosphatidylcholine and phosphatidylethanolamine in the mitochondria obtained a much higher level of radioactivity than that observed in experiments with the isolated mitochondria.

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