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Journal of Lipid Research, Vol. 1, 281-285, July 1960
Copyright © 1960 by Lipid Research, Inc.

Quantitative glass paper chromatography: a microdetermination of plasma cholesterol

Joseph R. Swartwout , Julius W. Dieckert , O. Neal Miller , and James G. Hamilton

The Department of Obstetrics and Gynecology, the Department of Biochemistry, and the Nutrition and Metabolism Research Laboratory of the Department of Medicine, Tulane University School of Medicine, New Orleans 12, Louisiana

A rapid, sensitive method for the determination of sterols is described. Microgram samples are spotted on impregnated glass fiber paper, chromatographed for 7 minutes, dried, sprayed with sulfuric acid, and charred by heating. The amount of char formed is measured by densitometry. Values obtained for cholesterol in the serum are in agreement with those obtained by the Sobel-Mayer method. The method has been adapted to the determination of cholesterol in 10 µl. of plasma, and to the determination of standard solutions of other sterols.

Submitted on February 2, 1960


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