J. Lipid Res.
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Journal of Lipid Research, Vol. 1, 349-351, July 1960
Copyright © 1960 by Lipid Research, Inc.

Rapid ultramicro estimation of serum total cholesterol

Ronald L. Searcy , Lois M. Bergquist , and Ralph C. Jung

Departments of Pathology, Los Angeles County Osteopathic Hospital, College of Osteopathic Physicians and Surgeons, Los Angeles 33, California

An ultramicro cholesterol technique based upon a new color reaction and requiring only 10 µl. of serum is proposed. The need for extraction with lipid solvent is eliminated by drying serum on filter paper segments and eluting directly in the ferrous sulfate color reagent. This method has been successfully applied to sera obtained from 200 blood donors.

Submitted on December 14, 1959


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