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Journal of Lipid Research, Vol. 10, 108-114, January 1969
Copyright © 1969 by Lipid Research, Inc.

Lipid composition of subcellular particles of human blood platelets

Aaron J. Marcus , Harris L. Ullman , and Lenore B. Safier

Hematology Section, New York Veterans Administration Hospital, New York 10010; and the Department of Medicine, Cornell University Medical College, New York 10021

Human platelets can be fractionated into three main subcellular components: granules, membranes, and a soluble fraction. In this study we determined the phospholipid and neutral lipid content of the granules and membranes. Quantitative relationships between lipids and protein were examined. The fatty acid and aldehyde composition of individual phospholipids and neutral lipids was also determined.

Whole platelets had a lower lipid to protein ratio than did the subcellular particles, but the basic lipid composition of the granules, membranes, and platelets was similar. The phospholipid composition of platelets and subcellular fractions was found to differ only in that granules had a lower percentage of lecithin. Each of the phospholipid classes displayed a distinctive fatty acid pattern which was the same in all fractions and in whole platelets.

The major neutral lipid was free cholesterol. Cholesteryl esters, triglycerides, and free fatty acids were minor components. The molar ratio of cholesterol to phospholipid in the platelet membranes was lower than that of brain myelin and erythrocyte ghosts. Some differences in fatty acid composition of the neutral lipids of platelet fractions were found.

A special lipid composition or constituent that would correlate with platelet function has not been found.

Supplementary key words cell membranes • free cholesterol • proteolipids • cardiolipin • cerebrosides

Submitted on July 30, 1968
Accepted on September 25, 1968

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