Journal of Lipid Research, Vol. 10, 33-40, January 1969
Copyright © 1969 by Lipid Research, Inc.
Separation of mono- and diglycerides by gas-liquid chromatography
Rodney Watts and R. Dils
Department of Biochemistry, University of Birmingham, Birmingham, England
The parameters affecting the separation and quantification of trimethylsilyl ethers of mono- and diglycerides have been investigated by gas-liquid chromatography with QF-1 and SE-30 as stationary phases and a flame ionization detector. Results have been compared with those obtained earlier for triglycerides.
The isothermal characteristics of a range of trimethylsilyl ethers of mono- and diglycerides on both stationary phases showed that log retention volume was directly proportional to carbon number and inversely proportional to absolute temperature. However, glyceride derivatives with lower carbon numbers deviated from these relationships.
By using various rates of programmed temperature rise, we have determined the elution temperatures (Kelvin scale) of the mono- and diglyceride trimethylsilyl ethers relative to that of glycerol trilaurate. The "carbon equivalent of a trimethylsilyl group" is defined and shown to be useful in comparing the chromatographic properties of different glyceride classes.
Weight and molar correction factors have been obtained and used to analyze diglycerides derived from egg and bovine brain lecithins.
Supplementary key words trimethylsilylation • isothermal • temperature programming • elution temperatures • correction factors
Submitted on June 24, 1968
Accepted on September 19, 1968
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