Journal of Lipid Research, Vol. 10, 47-55, January 1969
Copyright © 1969 by Lipid Research, Inc.

Separation and identification of ceramides derived from human plasma sphingomyelins

Bengt Samuelsson and Karin Samuelsson

Department of Medical Chemistry, Royal Veterinary College; Department of Neurology, Karolinska Sjukhuset; and Laboratory of Mass Spectrometry, Karolinska Institutet, Stockholm, Sweden

Sphingomyelins from human blood plasma have been converted into ceramides by enzymatic hydrolysis with phospholipase C. After acetylation the ceramides were fractionated by thin-layer chromatography on silica gel containing silver nitrate. Four main fractions obtained by this method were subsequently converted to di-O-trimethylsilyl ether derivatives and separated by gas-liquid chromatography on 1% OV-1. 2-11 components could be distinguished in each of the four fractions. The major fractions emerging from the gas chromatograph were analyzed by mass spectrometry and their main molecular species were identified. Two of the gas chromatographic fractions contained essentially pure molecular species, namely N-tetracosenoyl sphingosine and N-tetracosenoylsphinga-4, 14-dienine.

Supplementary key words phospholipase C • ceramide acetates • argentation chromatography • trimethylsilyl ethers • gas-liquid chromatography • mass spectrometry

Submitted on June 18, 1968
Accepted on August 16, 1968

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