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Journal of Lipid Research, Vol. 10, 83-90, January 1969
Copyright © 1969 by Lipid Research, Inc.

Effect of glucose feeding on net transport of plasma free fatty acids

Nome Baker and Hojat Rostami

Radioisotope Research, Veterans Administration Center, Los Angeles, California 90073, and Department of Biological Chemistry, University of California at Los Angeles School of Medicine, Los Angeles, California 90024

The effect of a single glucose feeding upon the net inflow and outflow transport of plasma free fatty acids (FFA) has been studied in 75 unanesthetized rats. The animals were fasted for 22 ± 2 hr; then 50 rats were refed 2 ml of 50% glucose by gastric intubation. At 0, 10-15, and 30-35 min after glucose refeeding, the rats were injected with palmitate-1-14C complexed to rat serum. The tracer dose included 131I-labeled albumin.

Plasma FFA concentration, 131I concentration, and FFA-14C were measured at five time intervals after injection of the tracer dose. From these data the irreversible disposal rate, or net outflow transport, and the net inflow transport of plasma FFA were calculated. Estimations were based upon a special case of a general solution for measuring net inflow and outflow transport of a circulating metabolite. The general solution is independent of the number of compartments, how they are interconnected, the number of nonradioactive inflows, and where the inflows enter the system.

Net inflow = net outflow transport = 7.6 µeq/min in the fasted state and 3.5 µeq/min in the new steady state that is reached 30-40 min after glucose refeeding. A very slight imbalance between the rates of net inflow and outflow transport could account for the rapid fall in plasma FFA concentration that results from a single glucose feeding. Theoretical and practical problems associated with studying inflow and outflow transport by means of the technique using a single injection of racer are discussed.

Supplementary key words irreversible disposal rate • inflow-outflow transport • nonsteady state • unanesthetized rats • palmitate turnover

Submitted on June 10, 1968
Accepted on August 15, 1968


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