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Journal of Lipid Research, Vol. 10, 175-178, March 1969
Copyright © 1969 by Lipid Research, Inc.

Esterification of injected epicoprostanol in a human subject

R. S. Rosenfeld and Leon Hellman

Institute for Steroid Research and the Division of Neoplastic Medicine, Montefiore Hospital and Medical Center, New York 10467

That a necessary requirement for in vivo esterification of 3-hydroxy sterols is the equatorial conformation of the hydroxyl group has been substantiated by the administration of epicoprostanol-3ßbeta;-3H (3-OH equatorial) to a man with a complete biliary fistula. Radioactive epicoprostanol was identified in both free and esterified sterols of plasma. The specific activity of biliary sterols was much higher than that of plasma sterols; this indicated a preferential removal of epicoprostanol from circulating sterols. The appearance of tritium in body water demonstrated that a portion of the labeled epicoprostanol underwent oxidation at C-3.

Supplementary key words 5ßbeta;-cholestan-3agr-ol • metabolism • equatorial configuration

Submitted on June 4, 1968
Accepted on November 13, 1968


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