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Journal of Lipid Research, Vol. 10, 179-182, March 1969
Copyright © 1969 by Lipid Research, Inc.

Absorption and lymphatic transport of cholesterol and sitosterol in the rat

Christer SylvÉN and Bengt BorgstrÖM

Division of Physiological Chemistry, Chemical Center, University of Lund, Lund, Sweden

An attempt was made to determine the mechanism for the greater absorbability of cholesterol as compared to sitosterol.

Sitosterol-22,23-³H in different combinations with cholesterol-4-¹⁴C, dissolved in 0.8 ml of triolein, was fed to rats with lymph fistulae. Feeding 1.5, 50, or 100 µmoles of sitosterol resulted in a transfer to the lymph in 24 hr of 3-6% of the sitosterol, largely independent of the dose fed. The total amount of sitosterol transferred to the lymph was therefore almost linearly related to the dose fed. 30% of a tracer dose of cholesterol-4-¹⁴C fed together with the sitosterol was transferred to the lymph in 24 hr.

When a total of 50 µmoles of sterol, containing cholesterol-¹⁴C and sitosterol-³H in the proportions 1:3, 1:1, and 3:1, was similarly fed, we found that sitosterol had no significant effect on the lymphatic transport of the simultaneously fed cholesterol.

The ratio of ³H to ¹⁴C in the lymph was between 0.1 and 0.2 (the ratio in each fed mixture being taken as 1.0). The ratio was constant during the absorption period and independent of the ratio of sterols in the fed sterol mixture. Thus the same percentage of each sterol was always absorbed, and the sterols exerted no mutual interference in each others' absorption.

We conclude that the mechanism for specificity in sterol absorption must be located early in the transport of the sterols within the intestinal mucosa cell.

Supplementary key words intestinal absorption • competition

Submitted on July 15, 1968
Accepted on November 4, 1968

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