HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by White, D. C. Articles by Tucker, A. N. Search for Related Content PubMed PubMed Citation Articles by White, D. C. Articles by Tucker, A. N. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 10, 220-233, March 1969
Copyright © 1969 by Lipid Research, Inc.

Phospholipid metabolism during bacterial growth

David C. White and Anne N. Tucker

Department of Biochemistry, University of Kentucky Medical Center, Lexington, Kentucky 40506

Haemophilus parainfluenzae incorporates glycerol and phosphate into the membrane phospholipids without lag during logarithmic growth. In phosphatidyl glycerol (PG), the phosphate and unacylated glycerol moieties turn over and incorporate radioactivity much more rapidly than does the diacylated glycerol. At least half the radioactivity is lost from the phosphate and unacylated glycerol in about 1 doubling. The total fatty acids turn over slightly faster than the diacyl glycerol.

In phosphatidyl ethanolamine (PE), which is the major lipid of the bacterium, ethanolamine and phosphate turn over and incorporate radioactivity at least half as fast as the phosphate in PG. The glycerol of PE did not turn over in 4 bacterial doublings. In phosphatidic acid the glycerol turns over at one-third the rate of phosphate turnover. By means of a modified method for the quantitative recovery of 1,3-glycerol diphosphate from cardiolipin, the phosphates and middle glycerol of cardiolipin were shown to turn over more rapidly than the acylated glycerols during bacterial growth. There is no randomization of the radioactivity in the 1- and 3-positions of the glycerol in the course of 1 doubling.

The fatty acids of PG turn over faster than those in PE. In both lipids the 2-fatty acids turn over much faster than the 1-fatty acids. At both positions the individual fatty acids have their own rates of turnover. The distribution of fatty acids between the 1- and 2-positions is the same as in other organisms, with more monoenoic and long-chain fatty acids at the 2-position.

The different rates of turnover and incorporation of radioactivity into different parts of the lipids suggest that exchange reactions may be important to phospholipid metabolism.

Supplementary key words turnover • incorporation • fatty acids • phospholipase A and C • phosphatidyl ethanolamine • phosphatidyl glycerol • cardiolipin • phosphatidic acid

Submitted on August 7, 1968
Accepted on November 27, 1968

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				C. Baysse, M. Cullinane, V. Denervaud, E. Burrowes, J. M. Dow, J. P. Morrissey, L. Tam, J. T. Trevors, and F. O'Gara Modulation of quorum sensing in Pseudomonas aeruginosa through alteration of membrane properties Microbiology, August 1, 2005; 151(8): 2529 - 2542. [Abstract] [Full Text] [PDF]