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Journal of Lipid Research, Vol. 10, 240-243, March 1969
Copyright © 1969 by Lipid Research, Inc.

Improved separation of sterols by reversed-phase thin-layer chromatography

Noel J. de Souza and William R. Nes

Department of Biological Sciences, Drexel Institute of Technology, Philadelphia, Pennsylvania 19104

Some closely related sterols have been separated with better resolution in a shorter period of time than has previously been reported. Separations were effected on the basis of carbon number and the number and location of double bonds through the use of paraffin-impregnated kieselguhr chromatoplates in the system (paraffin oil)/(acetone-water 4:1).

Supplementary key words phytosterols • cholesterol precursors

Submitted on July 25, 1968
Accepted on November 14, 1968


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