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Journal of Lipid Research, Vol. 10, 243-245, March 1969
Copyright © 1969 by Lipid Research, Inc.
Department of Laboratories, The Long Island Jewish Hospital, New Hyde Park, New York 11040
Phosphoglyceride phosphorus is readily determined directly on silica gel removed from thin-layer chromatograms, without elution, by a nondigestive procedure with a sulfuric-periodic acid reagent. The method is specific for hydrolyzable phosphoglyceride containing two acid-hydrolyzable groups. Cariolipin reacts only partially, while sphingomyelin, diether lecithin, and phosphonate cephalin fail completely to react.
Supplementary key words periodic-sulfuric acid nondigestive procedure
Submitted on April 2, 1968
Accepted on November 13, 1968
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