J. Lipid Res.
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Journal of Lipid Research, Vol. 10, 243-245, March 1969
Copyright © 1969 by Lipid Research, Inc.

Phosphorus determination in phosphoglycerides from thin-layer chromatograms

Arthur F. Rosenthal and Stella Ching-Hsien Han

Department of Laboratories, The Long Island Jewish Hospital, New Hyde Park, New York 11040

Phosphoglyceride phosphorus is readily determined directly on silica gel removed from thin-layer chromatograms, without elution, by a nondigestive procedure with a sulfuric-periodic acid reagent. The method is specific for hydrolyzable phosphoglyceride containing two acid-hydrolyzable groups. Cariolipin reacts only partially, while sphingomyelin, diether lecithin, and phosphonate cephalin fail completely to react.

Supplementary key words periodic-sulfuric acid • nondigestive procedure

Submitted on April 2, 1968
Accepted on November 13, 1968


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