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Journal of Lipid Research, Vol. 10, 245-246, March 1969
Copyright © 1969 by Lipid Research, Inc.

Improved method for determination of the position of double bonds in polyenoic fatty acid esters

J. N. Roehm and O. S. Privett

University of Minnesota, The Hormel Institute, Austin, Minnesota 55912

An improved method is described for determination of the position of double bonds in polyenoic fatty acid methyl esters. Partial reduction with hydrazine is employed without prior hydrolysis to the free fatty acid, and the resulting monoenes are identified by reductive ozonolysis.

Supplementary key words hydrazine reduction • ozonolysis • reductive gas-liquid chromatography

Submitted on September 12, 1968
Accepted on November 15, 1968

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