Journal of Lipid Research, Vol. 10, 253-259, May 1969
Copyright © 1969 by Lipid Research, Inc.

Effects of prolonged incubation and cell concentration on lipogenesis from glucose in isolated human omental fat cells

R. B. Goldrick , B. C. E. Ashley , and Margaret L. Lloyd

Department of Clinical Science, The John Curtin School of Medical Research, The Australian National University, Canberra, A. C. T. Australia

The behavior of human omental fat cells in vitro has been examined in order to define conditions under which glucose is converted to glyceride-glycerol and glyceride fatty acids.

Synthesis of glyceride fatty acids from glucose reached maximal rates only after several hours of incubation in Krebs-Henseleit bicarbonate buffer, with or without added bovine albumin. Conversion of glucose to glyceride fatty acids was readily demonstrable with concentrated cell suspensions and was stimulated 3- to 8-fold by insulin. With dilute cell suspensions, little fatty acid was synthesized even after prolonged incubation in the presence of insulin.

Conversion of glucose to glyceride-glycerol was linear during 6-hr incubations in buffer and unaffected by the concentration of the cell suspension. In the presence of bovine albumin, glyceride-glycerol synthesis was readily demonstrable at all cell concentrations used, although synthesis was faster in dilute suspensions.

Thus, different incubation conditions produce widely divergent patterns of glucose metabolism in human omental fat cells.

Supplementary key words glucose metabolism • buffer composition • insulin

Submitted on July 17, 1968
Accepted on October 31, 1968

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