Journal of Lipid Research, Vol. 10, 320-325, May 1969
Copyright © 1969 by Lipid Research, Inc.

Dehydration of prostaglandins: study by spectroscopic method

Niels H. Andersen

Converse Laboratory, Harvard University, Cambridge, Massachusetts 02138

The ultraviolet (UV) and optical rotatory dispersion (ORD) spectra of prostaglandins E₁, A₁, B₁, and their naturally derived 15-epimers are presented. The dehydration sequence E A B under acidic and basic conditions has been studied by UV and ORD. Conditions for quantitative conversion of PGE₁ to PGA₁ are described.

The combination of ORD and UV affords a nondestructive assay which can determine the relative amounts of E-, A-, and B-type prostaglandins with as little as 1-2 µg of material. The total quantity of prostaglandins can be estimated (±15%) in this way or, more accurately, by treatment with alkali of an aliquot (200 ng is sufficient) and determination of the change in absorbance at 278 nm.

Supplementary key words prostaglandins • spectroscopic assay • UV spectra • optical rotatory dispersion • ßbeta;-hydroxyketones

Submitted on July 15, 1968
Accepted on February 10, 1969

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