Journal of Lipid Research, Vol. 10, 341-344, May 1969
Copyright © 1969 by Lipid Research, Inc.

Simple liquid-liquid partition system for isolation of labeled oleic acid from mixtures with glycerides

Per Belfrage and Martha Vaughan

Molecular Disease Branch, National Heart Institute, National Institute of Health, Bethesda, Maryland 20014

Oleic acid has a partition coefficient, upper phase/lower phase, of 1.9 (22°C) in the liquid-liquid partition system described herein. Tri-, di-, and monoolein are found almost exclusively in the lower (organic) phase. Oleic acid can be quantitatively removed from mixtures of triglyceride and partial glycerides by means of this partition system under conditions resembling those in a lipase assay.

Supplementary key words triolein • diolein • monoolein

Submitted on October 14, 1968
Accepted on January 27, 1969

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