Journal of Lipid Research, Vol. 10, 363-369, July 1969
Copyright © 1969 by Lipid Research, Inc.

Sphingolipids in bean leaves (Phaseolus vulgaris)

H. E. Carter and J. L. Koob

Division of Biochemistry, Department of Chemistry and Chemical Engineering, University of Illinois, Urbana, Illinois 61801

Phytoglycolipid has been isolated for the first time from plant leaves (Phaseolus vulgaris). The purified product (almost identical with the phytoglycolipid isolated from flax seed) was a ceramide attached through phosphate diester linkage to an oligosaccharide, which consisted of the usual trisaccharide unit (inositol, hexuronic acid, hexosamine) to which were attached mannose, galactose, and arabinose. The major fatty acids were the saturated 2-hydroxy C₂₂, C₂₄, and C₂₆ acids; the major long-chain bases were dehydrophytosphingosine (d-ribo-1,3,4-trihydroxy-2-amino-8-trans-octadecene) (53%) and phytosphingosine (d-ribo-1,3,4-trihydroxy-2-amino-octadecane) (32%).

A ceramide and a cerebroside were also isolated. In the ceramide the major fatty acids and the major long-chain bases were the same as in the phytoglycolipid. In the cerebroside, the fatty acid composition was similar to that in the ceramide and phytoglycolipid, but the long-chain bases consisted of dehydrophytosphingosine and phytosphingosine (7:1) with a substantial amount of unidentified long-chain base. The sugar component was glucose.

Supplementary key words phytoglycolipid • cerebroside • ceramide • composition

Submitted on September 3, 1968
Accepted on February 7, 1969

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