J. Lipid Res.
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Journal of Lipid Research, Vol. 10, 460-462, July 1969
Copyright © 1969 by Lipid Research, Inc.

Dry-column chromatographic isolation of fatty acid esters of phorbol from croton oil

Paul R. Ocken

Department of Biochemistry, New Jersey College of Medicine and Dentistry, Jersey City, New Jersey 07304

The family of mixed fatty acid esters of 4,9,12,13,20-pentahydroxy-tiglia-1,6-dien-3-one (phorbol) has been isolated from croton oil by column chromatography on silica gel and preparative dry-column chromatography on silica gel HF/254 packed in a cellophane membrane. The tumorpromoting agents are obtained rapidly and with minimum difficulty.

Submitted on October 14, 1968
Accepted on March 25, 1969


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