Journal of Lipid Research, Vol. 10, 487-494, September 1969
Copyright © 1969 by Lipid Research, Inc.

Effect of glucose ingestion on the metabolism of free fatty acids in human subjects

C. Waterhouse , N. Baker , and H. Rostami

University of Rochester School of Medicine and Dentistry and Strong Memorial Hospital, Rochester, New York 14620; Radioisotope Research, Veterans Administration Center, Los Angeles, California 90073 and Department of Biological Chemistry, University of California School of Medicine, Los Angeles, California 90024

The rate of appearance of ¹⁴CO₂ in expired air after the injection of a single dose of NaH¹⁴CO₃ has been determined in normal individuals both in the fasted and fed states. These data were combined with previously obtained results on the rate of disappearance of injected palmitate-¹⁴C from the bloodstream, to give a multicompartmental analysis of free fatty acid oxidation and esterification.

The results confirm that glucose feeding promptly inhibits the rate of free fatty acid oxidation to CO₂. The "irreversible disposal rate," or irreversible flux of free fatty acids from the plasma, was also consistently reduced by glucose feeding. The diminution in irreversible disposal, not accounted for entirely by reduction of direct oxidation, must indicate suppression of other disposal mechanisms, including net esterification of free fatty acids. An average drop of 49% in "net esterification" when glucose was given may be compared with the 65% inhibition of rapid free fatty acid oxidation.

Supplementary key words compartmental analysis • bicarbonate injection • CO₂ excretion • free fatty acid oxidation • esterification • fasting • fed • human subjects

Submitted on September 27, 1968
Accepted on April 18, 1969

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