Journal of Lipid Research, Vol. 10, 507-514, September 1969
Copyright © 1969 by Lipid Research, Inc.

Synthesis of lipids from acetate by human preputial and abdominal skin in vitro

Hugh E. Vroman , R. A. Nemecek , and S. L. Hsia

Departments of Dermatology and Biochemistry, University of Miami School of Medicine, Miami, Florida 33136

Lipogenesis in vitro from acetate-1-¹⁴C was studied in human preputial skin and abdominal skin. Radioactive lipids were separated by column chromatography on Florisil and by thin-layer chromatography on silica gel. Radioactivity was incorporated chiefly into the triglyceride, sterol, and polar lipid fractions, while lesser amounts of ¹⁴C were found in the hydrocarbon, wax, diglyceride, monoglyceride, and fatty acid fractions; labeling of steryl esters was minimal. On thin-layer chromatography, the radioactive polar lipids had mobilities similar to lysolecithin, phosphatidyl choline, phosphatidyl ethanolamine, and phosphatidic acid.

The radioactive fatty acids of the different lipid fractions were separated by gas-liquid chromatography. The major ¹⁴C-labeled acids were 16:0 and 18:0. Radioactivity was also detected in acids 14:0, 15:0, 16:1, 18:1, 18:2, 20:0, 20:1, 22:0, 24:0, 24:1, and 26:0. No radioactivity could be detected in arachidonic acid, although this fatty acid comprises 9% of the chromatographed fatty acids. The pattern of incorporated ¹⁴C was different from the percentage mass composition of the fatty acids.

Skin is therefore active in the biosynthesis of a wider variety of lipids than previously demonstrated.

Supplementary key words lipogenesis • triglyceride • free sterols • phosphoglycerides • squalene • diglycerides • monoglycerides • fatty acid composition • waxes • steryl esters

Submitted on January 30, 1969
Accepted on May 1, 1969

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				V. A Ziboh, C. C Miller, and Y. Cho Metabolism of polyunsaturated fatty acids by skin epidermal enzymes: generation of antiinflammatory and antiproliferative metabolites1 Am. J. Clinical Nutrition, January 1, 2000; 71(1): 361S - 366S. [Abstract] [Full Text] [PDF]