Journal of Lipid Research, Vol. 10, 577-588, September 1969
Copyright © 1969 by Lipid Research, Inc.
Isolation and properties of lipoproteins from normal rat serum
Shunichi Koga , David L. Horwitz , and Angelo M. Scanu
Departments of Medicine and Biochemistry, The University of Chicago, and Argonne Cancer Research Hospital, Chicago, Illinois 60637
Three major classes of lipoproteins (VLDL, d < 1.006; LDL, d 1.006-1.040; HDL, d 1.063-1.21) were isolated by ultracentrifugal flotation from the serum of normal male Sprague-Dawley rats. Their physical, chemical, and immunological properties were analyzed and compared with those of their water-soluble, essentially lipid-free derivatives. Studies were also carried out on the d > 1.21 fractions. Each product was found to have distinct characteristics, and this was also indicated by spectral analyses carried out by the techniques of circular dichroism and UV absorption spectroscopy. The results provided evidence for the mutual role of the protein and lipids in determining the structure, and perhaps the immunological specificity, of serum lipoproteins.
Supplementary key words apoproteins • immunology • disc gel electrophoresis • circular dichroism • UV absorption • lipoprotein structure
Submitted on March 10, 1969
Accepted on June 23, 1969
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
