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Journal of Lipid Research, Vol. 10, 599-608, September 1969
Copyright © 1969 by Lipid Research, Inc.

Relation of mitochondrial phospholipase A activity to mitochondrial swelling

Moseley Waite , L. L. M. Van Deenen , T. J. C. Ruigrok , and P. F. Elbers

Department of Biochemistry, The Bowman Gray School of Medicine of Wake Forest University, Winston-Salem, North Carolina 27103, and Department of Biochemistry and the Biological Ultrastructure Research Unit, The State University of Utrecht, Utrecht, The Netherlands

Assays of mitochondrial phospholipase A activity and mitochondrial swelling demonstrated that the phospholipase A activity is related to the swelling under the experimental conditions used. Both were stimulated by added free fatty acid and CaCl2, not affected greatly by the addition of monoacyl phosphoglycerides, and inhibited by EDTA. The amount of fatty acid hydrolyzed from endogenous phosphatidyl ethanolamine and phosphatidyl choline during swelling was calculated to be 20-30 times less than the amount of added free fatty acid that gave comparable swelling. Under the experimental conditions about 4% of the phospholipid was hydrolyzed.

Mitochondrial swelling was studied by electron microscopy and turbidity measurements. The results found were in agreement, whether oleic acid was present or not, except for those values obtained after very brief incubation (1 min) and after incubation for longer than 35 min.

The lack of direct proportion between swelling and the concentration of lysosomes present indicated that the swelling is related mainly to mitochondrial phospholipase A, although swelling due to contaminating lysosomes cannot be excluded entirely.

The temperature dependence of spontaneous, fatty acid-induced, or CaCl2-induced swelling suggested that enzymatic activities are responsible for swelling.

Supplementary key words correlation • phospholipase A • swelling • oleic acid • CaCl2 • EDTA • electron microscopy • temperature dependence

Submitted on October 8, 1968
Accepted on April 29, 1969


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