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Journal of Lipid Research, Vol. 10, 614-616, September 1969
Copyright © 1969 by Lipid Research, Inc.

One-step quantitative extraction of medium-chain and long-chain fatty acids from aqueous samples

Manley Cohen , R. G. H. Morgan , and Alan F. Hofmann

Mayo Graduate School of Medicine and Gastroenterology Unit, Mayo Clinic and Mayo Foundation, Rochester, Minnesota 55901

Medium-chain (C₆ and longer) fatty acids, as well as 12-hydroxystearic and long-chain fatty acids, can be quantitatively extracted into toluene and titrated in the toluene phase with tetrabutylammonium hydroxide. The method may be useful in determinations of fecal and serum fatty acids and of the products of lipolysis.

Supplementary key words medium-chain triglycerides • free fatty acid • titration • octanoic acid • 12-hydroxystearic acid • feces • serum • lipolysis

Submitted on February 24, 1969
Accepted on June 4, 1969

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