Journal of Lipid Research, Vol. 10, 660-667, November 1969
Copyright © 1969 by Lipid Research, Inc.

Improved quantitation of plasma lipids by direct gas-liquid chromatography

A. Kuksis , O. Stachnyk , and B. J. Holub

Banting and Best Department of Medical Research and the Department of Biochemistry, University of Toronto, Toronto, Canada

A preliminary digestion of total plasma lipid extracts with phospholipase C, which converts the lysolecithins, lecithins, and sphingomyelins into monoglycerides, diglycerides, and ceramides, respectively, has been shown to facilitate subsequent determination of the plasma lipids by gas-liquid chromatography. A further improvement in the chromatographic elution pattern results from acetylation or trimethylsilylation of the liberated alcohol moieties prior to injection into the chromatograph. If tridecanoin is used as internal standard, quantitative estimates can be rapidly obtained for plasma lysolecithins, free cholesterol, lecithins, sphingomyelins, cholesteryl esters, and triglycerides, as well as for free fatty acids. Other plasma lipids do not occur in sufficiently high concentrations to interfere with the analysis. The determination requires 0.1-0.5 ml of plasma and about 6 hr of processing, but many samples can be processed at a time.

Supplementary key words phospholipase C • lysolecithins • monoglycerides • lecithins • diglycerides • sphingomyelins • ceramides • trimethylsilyl ethers • acetates • trifluoroacetates

Submitted on February 24, 1969
Accepted on July 14, 1969

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