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Journal of Lipid Research, Vol. 10, 668-673, November 1969
Copyright © 1969 by Lipid Research, Inc.

Hepatic acetoacetyl-CoA deacylase activity in rats fed ethyl chlorophenoxyisobutyrate (CPIB)

Robert E. Burch and George L. Curran

Francis Delafield Hospital, and the Department of Medicine, College of Physicians and Surgeons, Columbia University, New York 10032

Intact or sonicated mitochondria from the livers of rats fed a diet containing 0.2% ethyl chlorophenoxyisobutyrate (CPIB) for 3 wk showed acetoacetyl-CoA deacylase activity enhanced 26 and 39%, respectively, over that shown by comparable fractions from rats fed the same diet without CPIB. The corresponding supernatant fractions did not differ in activity. The enhanced activity of mitochondrial acetoacetyl-CoA deacylase in the livers of the CPIB-treated rats could effectively decrease the amount of acetoacetyl CoA available within the cell for synthetic processes.

Supplementary key words cholesterol biosynthesis • acetoacetate • HMG-CoA

Submitted on April 24, 1969
Accepted on July 16, 1969


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