Journal of Lipid Research, Vol. 11, 38-41, January 1970
Copyright © 1970 by Lipid Research, Inc.

Stimulation of lipolysis in adipose tissue in vitro by inhibitors of lipid mobilization

Floyd P. Kupiecki and Diana I. Schneider

Department of Diabetes Research, The Upjohn Company, Kalamazoo, Michigan 49001

5-Methylpyrazole-3-carboxylic acid (U-19425) and nicotinic acid, which apparently inhibit lipolysis in vivo as indicated by low plasma FFA and glycerol concentrations, stimulate lipolysis in vitro in adipose tissue removed from fasted rats 30-90 min after treatment. This stimulation is not the result of low initial levels of FFA in adipose tissue. An increased rate of lipolysis is not induced in vitro by preincubating tissue of untreated rats with U-19425. The increase can be blocked in incubated adipose tissue of U-19425-treated animals if U-19425 is added to the incubation medium. The ßbeta;-adrenergic blocking agent propranolol, at a concentration which nhibits epinephrine-stimulated lipolysis, does not affect the increase produced by administration of U-19425.

Supplementary key words 5-methylpyrazole-3-carboxylic acid (U-19425) • nicotinic acid • plasma and tissue FFA • glycerol • epinephrine • propranolol

Submitted on January 20, 1969
Revised on May 26, 1969
Accepted on October 3, 1969

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