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Journal of Lipid Research, Vol. 11, 118-123, March 1970
Copyright © 1970 by Lipid Research, Inc.

Determination of -tocopherol in erythrocytes by gas-liquid chromatography

J. G. Bieri , R. K. H. Poukka , and E. L. Prival

Laboratory of Nutrition and Endocrinology, National Institute of Arthritis and Metabolic Diseases, National Institutes of Health, Bethesda, Maryland 20014

A method is described for the determination of submicrogram amounts of -tocopherol in 0.5 ml of packed erythrocytes. The -tocopherol in a lipid extract is oxidized to -tocopherylquinone which is separated by thin-layer chromatography, eluted, and quantitated by gas-liquid chromatography. Calculation is based on the recovery of added -tocopherol-³H.

Erythrocytes from stock rats had an average -tocopherol concentration of 344 µg/100 ml of packed cells, while for human cells the average was 235 µg/100 ml. The ratio of red cell to plasma -tocopherol was 0.482 for rat blood, and 0.244 for human blood.

Supplementary key words erythrocyte -tocopherol • red cell vitamin E • blood -tocopherol • vitamin E determination

Submitted on August 21, 1969
Revised on November 10, 1969
Accepted on December 8, 1969

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