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Journal of Lipid Research, Vol. 11, 144-149, March 1970
Copyright © 1970 by Lipid Research, Inc.

Preparation of radioactive Tay-Sachs ganglioside labeled in the sialic acid moiety

Edwin H. Kolodny , Roscoe O. Brady , Jane M. Quirk , and Julian N. Kanfer

Laboratory of Neurochemistry, National Institute of Neurological Diseases and Stroke, National Institutes of Health, Bethesda, Maryland 20014

A procedure is described for the preparation of Tay-Sachs ganglioside specifically labeled in the sialic acid portion of the molecule. Rat brain gangliosides were labeled biosynthetically by the intracranial injection of N-acetyl-³H-d-mannosamine. Radioactive gangliosides were isolated and selectively degraded with bacterial neuraminidase and rat liver ßbeta;-galactosidase to Tay-Sachs ganglioside-³H. Radioactivity in the labeled product was confined to the N-acetyl-neuraminic acid portion of the molecule.

Supplementary key words biosynthesis • N-acetyl-³H-d-mannosamine • neuraminidase • ßbeta;-galactosidase • sialic acid-³H

Submitted on September 2, 1969
Accepted on December 15, 1969

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