Journal of Lipid Research, Vol. 11, 150-157, March 1970
Copyright © 1970 by Lipid Research, Inc.

Gas-liquid chromatography-mass spectrometry of synthetic ceramides containing 2-hydroxy acids

Sven Hammarström , Bengt Samuelsson , and Karin Samuelsson

Department of Medical Chemistry, Royal Veterinary College, Department of Neurology, Karolinska sjukhuset, Stockholm, Sweden

Ceramides containing either sphingosine or sphinganine and one of the 2-hydroxy acids, 14h:0, 16h:0, 18h:0, 20h:0, 22h:0, 24h:0, and 26h:0 were prepared and separated by gas chromatography as the 1,3,2'-tri-O-tri-methylsilyl derivatives. Mass spectrometric analyses of these derivatives showed that the ions formed on electron impact can be used to determine unequivocally the structures of the long-chain base and the fatty acid residue in the ceramide. Proposed structures of ions and the mechanisms of reaction of their formation are supported by mass spectra of homologous derivatives, by deuterium labeling experiments, and by high-resolution on mass spectrometry.

Supplementary key words trimethylsilyl ethers • sphingosine • sphinganine • deuterium labeling • silicic acid chromatography

Submitted on May 16, 1969
Accepted on November 5, 1969

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				F. C. Nichols Novel ceramides recovered from Porphyromonas gingivalis: relationship to adult periodontitis J. Lipid Res., December 1, 1998; 39(12): 2360 - 2372. [Abstract] [Full Text]