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Journal of Lipid Research, Vol. 11, 164-166, March 1970
Copyright © 1970 by Lipid Research, Inc.

Fluorimetric determination of sphingosine and its application to natural mixtures of glycosphingolipids

L. Coles and G. M. Gray

The Lister Institute of Preventive Medicine, Chelsea Bridge Road, London, S. W. 1, England

A sensitive estimation of sphingosine, by measurement of the fluorescence of a complex formed with 1-naphthylamino-4-sulfonic acid, is described. The practical range is 5-35 nmoles sphingosine. The method is used to estimate, in terms of sphingosine, amounts of ceramide and glycosphingolipids. The isolation of microamounts (5-30 µg) of individual glycosphingolipids from a mixture, and their quantitative estimation is described. The percentage composition of a glycosphingolipid mixture from the kidneys of adult C57/BL male mice is given.

Supplementary key words glycosyl ceramides • sulfatide • thin-layer chromatography • mouse kidney

Submitted on August 14, 1969
Accepted on December 1, 1969

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