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Journal of Lipid Research, Vol. 11, 231-236, May 1970
Copyright © 1970 by Lipid Research, Inc.

Use of ³H-labeled triether, a nonabsorbable oil-phase marker, to estimate fat absorption in rats with cholestyramine-induced steatorrhea

Reginald G. H. Morgan and Alan F. Hofmann

The Gastroenterology Unit, Mayo Clinic and Mayo Foundation, Rochester, Minnesota 55901

A tritium-labeled glycerol triether was tested as a nonabsorbable oil-phase marker in studies of fat absorption in normal rats and in rats with steatorrhea induced by various doses of cholestyramine. Animals were fed a test meal containing ³H-labeled triether and ¹⁴C-labeled trilinolein. Fat absorption was estimated in the following three ways: (a) by isotope ratios (the change in ³H/¹⁴C in the test meal and in feces); (b) by isotope recovery (the total fecal excretion of ¹⁴C radioactivity); and (c) by chemical recovery (the total fecal fat excretion). Absorption calculated from isotope ratios agreed well with that calculated from isotope recovery over a range of fat absorption of 50-100%, thus validating the use of this lipid marker under these conditions of fat malabsorption. Absorption calculated from chemical recovery was consistently poorer than that calculated from isotope ratios or isotope recovery, thus suggesting that cholestyramine increased the excretion of nondietary (endogenous) fat. Triether may be of value for studying the absorption of compounds present predominantly in the oil phase during digestion and may have significant advantages over other proposed lipid markers.

Supplementary key words glycerol triether • ether lipid metabolism • ³H-labeled glycerol triether • 1-hexa-decyl-2,3-didodecyl glycerol • 1-hexadecoxy-2,3-didodecoxy-propane

Submitted on October 2, 1969
Accepted on January 26, 1970

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