Journal of Lipid Research, Vol. 11, 293-300, July 1970
Copyright © 1970 by Lipid Research, Inc.

Computer treatment of gas-liquid chromatographic data, with special reference to fatty acid methyl esters

A. W. Boyne and W. R. H. Duncan

Rowett Research Institute, Bucksburn, Aberdeen, AB2 9SB, Great Britain

A computer program is described which analyzes output punched directly onto paper tape from a gas-liquid chromatograph. Although this program was written specifically for samples of fatty acid methyl esters derived from adipose tissue triglycerides which are eluted within 1 hr, modification of the dimension statements in the program would enable it to deal with samples which require a longer time to come off the column.

The salient features of the rationale of the program are discussed in detail, particularly the procedures for base line correction and for estimating the contributions from components which are not perfectly separated in the column. Examples are given of the program in practice, of comparing the results it gives with those obtained by manual triangulation of the areas on a recorder chart, and of indicating the range of column load over which we have found that it operates satisfactorily. A sample computer print-out from the program is presented and interpreted.

Supplementary key words carbon number • methyl palmitate • methyl stearate • triangulation • adipose tissue

Submitted on December 16, 1969
Accepted on March 12, 1970

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