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Journal of Lipid Research, Vol. 11, 311-321, July 1970
Copyright © 1970 by Lipid Research, Inc.

Sequential enzymes of linoleic acid oxidation in corn germ: lipoxygenase and linoleate hydroperoxide isomerase

H. W. Gardner

Northern Regional Research Laboratory, Peoria, Illinois 61604

Linoleic acid oxidation catalyzed by lipoxygenase (lipoxidase) activity in extracts of defatted corn germ does not terminate in the product, linoleic acid hydroperoxide, unless the lipoxygenase is first partially purified. If purification is not attempted, the hydroperoxide product exists only as a barely detectable intermediate in the synthesis of three products. One of these was identified as 9-hydroxy-10-oxo-cis-12-octadecenoic acid formed from the hydroperoxide by the enzyme, linoleate hydroperoxide isomerase. Another product, 13-hydroxy-10-oxo-trans-11-octadecenoic acid, is believed to be formed by an isomerase also. The third product was the linoleate ester of one of the hydroxy-oxo-fatty acids, 9-(cis-9,cis-12-octadecadienoyl)-10-oxo-cis-12-octadecenoic acid. It is not known if the synthesis of the ester is enzyme-catalyzed.

When a mixture of 13-hydroperoxy-cis-9,trans-11-octa-decadienoic acid and 9-hydroperoxy-trans-10,cis-12-octa-decadienoic acid from soybean lipoxygenase oxidation of linoleic acid was used as a substrate, 13-hydroxy-12-oxo-cis-9-octadecenoic acid and 9-hydroxy-12-oxo-trans-10-octadecenoic acid were formed as the major products of catalysis by linoleate hydroperoxide isomerase(s) from corn. Smaller quantities of 9-hydroxy-10-oxo-cis-12-octadecenoic acid and 13-hydroxy-10-oxo-trans-11-octadecenoic acid were also formed.

Supplementary key words linoleic acid hydroperoxide • 9-hydroxy-10-oxo-cis-12-octadecenoic acid • 13-hydroxy-12-oxo-cis-9-octadecenoic acid • 9-(cis-9, cis-12-octadecadienoyl)-10-oxo-cis-12-octadecenoic acid • 9-hydroxy-12-oxo-trans-10-octadecenoic acid • 13-hydroxy-10-oxo-trans-11-octadecenoic acid

Submitted on July 28, 1969
Accepted on March 17, 1970


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