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Journal of Lipid Research, Vol. 11, 378-381, July 1970
Copyright © 1970 by Lipid Research, Inc.

Thin-layer chromatography of tissue lipids without extraction

George D. Cherayil and K. S. Scaria

Department of Pathology, Marquette School of Medicine, Milwaukee, Wisconsin 53233, and Department of Biology, Mount Saint Paul College, Waukesha, Wisconsin 53186

Frozen tissue sections were applied directly to silica gel plates, and the lipids were separated by developing the plates in different solvent systems. Quantitation of the lipid classes was achieved by direct transmission densitometry after the plates were treated with a chromic acid-sulfuric acid spray.

Supplementary key words histchromatography • brain lipids • adipose tissue • gangliosides • cholesterol • phospholipids • neutral lipids

Submitted on October 14, 1969
Accepted on March 17, 1970


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