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Journal of Lipid Research, Vol. 11, 381-385, July 1970
Copyright © 1970 by Lipid Research, Inc.

Separation by gel chromatography of naturally occurring phosphatidylcholine mixtures according to number of ethylenic linkages

Richard J. King and John A. Clements

Cardiovascular Research Institute, University of California, San Francisco Medical Center, San Francisco, California 94122

This paper describes a procedure for the separation of lecithins according to the number of ethylenic bonds in their fatty acid residues. The procedure uses a column of alkylated dextran (Sephadex LH-20) eluted with an organic solvent system, the unsaturated lipids being separated as their mercuric acetate addition compounds. The system is capable of resolving at least four species of lecithin, and the intact lecithin molecules can be recovered for further study. The chromatographic system has been tested with lecithin derived from dog lung, rat liver, and hen's egg.

Supplementary key words alveolar surface active material • Sephadex LH-20 • phosphatidylcholine chromatography

Submitted on September 24, 1969
Accepted on March 17, 1970


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