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Journal of Lipid Research, Vol. 11, 386-388, July 1970
Copyright © 1970 by Lipid Research, Inc.

Novel preparation of cardiolipin from beef heart

Joseph Eichberg and John D. Burnham

McLean Hospital, Belmont, Massachusetts 02178; and Department of Biological Chemistry, Harvard Medical School, Boston, Massachusetts 02115

A new method is described for the isolation of beef heart cardiolipin. A lipid-protein complex, rich in cardiolipin, is obtained by a one-step solvent fractionation of the tissue total lipid extract. Cardiolipin in the complex is largely freed of protein by salt denaturation and is further purified by gel filtration on Sephadex LH-20 followed by column chromatography on bicarbonate-treated silicic acid. The highly purified product is obtained as the sodium salt in a yield of 85-100 mg/100 g of fresh tissue.

Supplementary key words Sephadex LH-20 • gel filtration • proteolipid

Submitted on January 13, 1970
Accepted on April 3, 1970

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