Journal of Lipid Research, Vol. 11, 496-498, September 1970
Copyright © 1970 by Lipid Research, Inc.

Preparation of labeled alkyl acyl glycerophosphorylcholine

A. Poulos

Department of Chemical Pathology, The National Hospital, Queen Square, London, England

A method for the preparation of 1-alkyl 2-acyl glycero-3-phosphorylcholine, labeled in the 2-position with oleic acid-1-¹⁴C is described. This method, based on the acylation of a 1-alkyl glycero-3-phosphorylcholine-CdCl₂ complex which is, in turn, formed from 1-alk-1-enyl glycero-3-phosphorylcholine by hydrogenation and subsequent reaction with cadmium chloride, offers a relatively simple means of preparing a labeled saturated ether phospholipid suitable for use in metabolic studies.

Supplementary key words phosphatidalcholine • 1-alkyl 3-glycero-3-phosphorylcholine-CdCl₂ complex • acylation • saturated ether phospholipid • lecithinase C hydrolysis

Submitted on December 23, 1969
Accepted on May 8, 1970

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