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Journal of Lipid Research, Vol. 11, 498-499, September 1970
Copyright © 1970 by Lipid Research, Inc.

An improved spray reagent for detection of bile acids on thin-layer chromatoplates

Thomas F. Kellogg

Department of Microbiology and Lobund Laboratory, Biochemistry and Biophysics Program, University of Notre Dame, Notre Dame, Indiana 46556

A spray reagent containing 8-hydroxy-1,3,6-pyrenetrisulfonic acid sodium salt is described, which allows detection of as little as 1 µg of bile acids under long-wave UV light. The bile acids can be eluted directly from the sprayed plates with acetone without eluting the spray reagent.

Supplementary key words 8-hydroxy-1,3,6-pyrenetrisulfonic acid • ultraviolet light

Submitted on April 30, 1970
Accepted on July 1, 1970


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