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Journal of Lipid Research, Vol. 11, 609-612, November 1970
Copyright © 1970 by Lipid Research, Inc.

Simplified fluorometric method for the determination of plasma glycerol

Mayer B. Davidson and Robert Karjala

Department of Medicine, University of California at Los Angeles School of Medicine, and Wadsworth Veterans Administration Hospital, Los Angeles, California 90024

A simplified method for determining plasma glycerol is described. This assay utilizes the fluorometric measurement of the reduced adenine dinucleotide, NADH2 which is formed when glycerol is oxidized by glycerol dehydrogenase. Only three pipettings are necessary for each reaction tube, and a large number of samples can be included in each assay.

Supplementary key words glycerol • glycerol dehydrogenase • NADH2

Submitted on May 18, 1970
Accepted on August 7, 1970


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