Journal of Lipid Research, Vol. 12, 17-23, January 1971
Copyright © 1971 by Lipid Research, Inc.

Metabolism of cholestane-3ßbeta;,5,6ßbeta;-triol. II. Identification of two major neutral metabolites in the rat

Henry G. Roscoe and Marvin J. Fahrenbach

Department of Metabolic Chemotherapy, Experimental Therapeutics Research Section, Lederle Laboratories Division, American Cyanamid Company, Pearl River, New York 10965

Rats were given a single oral dose of cholestane-3ßbeta;,5,6ßbeta;-triol-4-¹⁴C, and their feces were collected. The two major neutral metabolites were separated and isolated by use of solvent fractionation and chromatographic methods. The metabolites were identified as cholestane-3ßbeta;,5-diol-6-one and a mixture of long-chain fatty acid esters of cholestane-3ßbeta;,5,-6ßbeta;-triol. Cholestane-3ßbeta;,5-diol-6-one was identified using thin-layer and gas-liquid chromatography, infrared spectroscopy, and the spectrum produced by reaction with 65% sulfuric acid. The mixed esters of cholestane-3ßbeta;,5,6ßbeta;-triol were subjected to basic hydrolysis, and the steroid moiety was identified using the same techniques employed for cholestane-3ßbeta;,5-diol-6-one. The fatty acids were analyzed by gas-liquid chromatography of their methyl esters.

Supplementary key words cholestane-3ßbeta;,5,diol-6-one • fatty acids • sterol esters

Submitted on June 3, 1970
Accepted on September 14, 1970

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