Journal of Lipid Research, Vol. 12, 24-30, January 1971
Copyright © 1971 by Lipid Research, Inc.
The composition of marine-oil triglycerides as determined by silver ion-thin-layer chromatography
Nestor R. Bottino
Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas 77843
The fractionation of marine-oil triglycerides according to their degree of unsaturation was achieved on silica gel thin layers with 8% silver nitrate (w/w). Under the optimum conditions, cod-liver and whale oils were reproducibly separated into seven and six fractions, respectively. The fatty acid compositions of the fractions obtained from cod-liver and whale oils were further studied by gas-liquid chromatography. The following was found in both oils: Saturated and monoenoic acids were not only abundant in their corresponding fractions, but also comprised about two-thirds of the fatty acids in the more unsaturated fractions. Instead, polyenoic fatty acids of similar degrees of unsaturation predominated only in the particular fraction which corresponded to their number of double bonds. Thus, the distribution of fatty acids of varying degrees of unsaturation among marine triglycerides is not random.
Supplementary key words highly unsaturated triglycerides • polyunsaturated fatty acids
Submitted on October 20, 1969
Revised on July 13, 1970
Accepted on September 15, 1970
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