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Journal of Lipid Research, Vol. 12, 143-148, March 1971
Copyright © 1971 by Lipid Research, Inc.

Composition of human cerebrospinal fluid cerebroside

Yoshitaka Nagai and Julian N. Kanfer

Neurology Research and Joseph P. Kennedy, Jr. Memorial Laboratories, Massachusetts General Hospital, Boston, Massachusetts 02114

A technique was developed to isolate sufficient material for compositional analysis of cerebroside from pooled human cerebrospinal fluid. The carbohydrate moiety was principally galactose. The sphingosine base and fatty acid compositions were found to be similar to that of brain cerebroside. The presence of a contaminant in commercial silica gel which chromatographed like the trimethylsilyl derivative of glucose is described.

Supplementary key words sphingolipid isolation

Submitted on July 30, 1970
Accepted on October 28, 1970

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