Journal of Lipid Research, Vol. 12, 192-197, March 1971
Copyright © 1971 by Lipid Research, Inc.

Reduction and esterification of cholesterol and sitosterol by homogenates of feces

R. S. Rosenfeld and Leon Hellman

Institute for Steroid Research and Department of Oncology, Montefiore Hospital and Medical Center, New York 10467

Mixtures of cholesterol-1,2-³H and sitosterol-4-¹⁴C have been incubated with suspensions of feces in order to compare the behavior of the phytosterol with transformations known to take place with cholesterol under these conditions. Within the limitations of the study, both labeled sterols were esterified to the same extent, and reduction of the ⁵ double bond to the saturated analogue proceeded equally in both substances. After correcting for procedural losses, the recoveries of ³H and ¹⁴C from the incubations were always less than the controls; this strongly indicates destruction of sterol by feces microorganisms.

Supplementary key words feces sterols

Submitted on June 1, 1970
Accepted on November 13, 1970

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