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Journal of Lipid Research, Vol. 12, 221-232, March 1971
Copyright © 1971 by Lipid Research, Inc.

An evaluation of four methods for measuring cholesterol absorption by the intestine in man

Eder Quintão , Scott M. Grundy , and E. H. Ahrens Jr.

The Rockefeller University, New York 10021

Critical comparisons have been made in 12 patients of four methods for measuring cholesterol absorption from the intestine. Methods I-III depend on the use of labeled cholesterol (intravenously or continuous labeling orally) in conjunction with sterol balance measurements; Method IV can be carried out with only a single test dose containing labeled cholesterol plus labeled ßbeta;-sitosterol. In the latter technique absorption is calculated as the loss of cholesterol relative to ßbeta;-sitosterol during intestinal transit.

Method III (isotopic steady-state method) proved to be undependable because of uncertainties in determining the existence of an isotopic steady state. However, Method IV gave good agreement with Methods I and II, and it appears to have certain practical as well as theoretical advantages. Although Method IV requires collections of stools for up to 8 days, it is nevertheless the most rapid and the simplest of all the methods for estimating absorption. It can also be used in certain situations, such as in fur-licking animals, when Methods I and II are inadequate. Therefore, this method would seem to be a valuable addition to other isotopic techniques for estimating cholesterol absorption in man.

Supplementary key words cholesterol-4-¹⁴C • ßbeta;-sitosterol-22,23-³H • isotopic steady state • sterol balance method • isotope kinetics • fur-licking animals

Submitted on August 21, 1970
Accepted on December 8, 1970

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