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Journal of Lipid Research, Vol. 12, 257-259, March 1971
Copyright © 1971 by Lipid Research, Inc.
Biochemical Laboratory, Department of Pathobiology, School of Public Health and Community Medicine, University of Washington, Seattle, Washington 98105
The quantitative isolation of total glycosphingolipids from crude lipid extracts without contamination from other lipid classes is described. The method consists of (a) acetylation of total lipids with pyridine and acetic anhydride, (b) separation of acetylated glycolipids from nonglycolipids on a magnesia-silica gel (Florisil) column, and (c) deacetylation of glycolipid in chloroform-methanol-sodium methoxide. This method is useful for determination of microgram quantities of glycolipids derived from less than 1 ml of packed cells.
Supplementary key words glycolipids • acetylation • acetic anhydride • pyridine • magnesia-silica gel (Florisil) • 1,2-dichloroethane (DCE) • acetone • deacetylation
Submitted on August 7, 1970
Accepted on October 28, 1970
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