Journal of Lipid Research, Vol. 12, 265-269, May 1971
Copyright © 1971 by Lipid Research, Inc.

Agarose-starch gel electrophoresis of rat serum lipoproteins

A. Chalvardjian

Research Institute, The Hospital for Sick Children, Toronto 101, Ontario, Canada

Rat serum lipoproteins were separated into at least four fractions by agarose-starch gel electrophoresis. The system used was discontinuous in that glycine and sodium barbitone buffer was used in the reservoirs and Tris buffer was used for the gels. The four major bands could be related to the pattern obtained by ultracentrifugation. The high density lipoproteins consisted of at least two poorly resolved bands and were not separated from albumin.

The vertical gel apparatus was further modified to accept 0.4 ml of rat plasma, which was prestained with Sudan black. After electrophoresis the different lipoprotein bands could conveniently be cut out and the lipid phosphorus determined. The addition of Sudan black B decreased the recovery of the low and high density lipoproteins by 5-9%. However, the recovery of phospholipids was reproducible (80 ± 2%) and the high density lipoproteins contained over two-thirds of the plasma lipid phosphorus.

Supplementary key words discontinuous system • analytical, preparative, ultracentrifugal correlation • prestained

Submitted on June 1, 1970
Accepted on December 14, 1970

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